CRAMP
Digital Camera Transects
Field
Images
From 1998
through 2004 video data was taken using a Sony DCR-TRV900 Mini DV camcorder
enclosed in an Amphibico VHDB0900 Dive Buddy Housing. During early 2000 we
added a Quest Aqua-Lite dual head U/W video light system later updating to
NiteRider divelights.
Will Smith of the UH Dept. of Geography running a video
transect. Photo by Paul Jokiel.
CRAMP has
since improved its methodology to keep up with advances in technology by
replacing video with digital stills. Unlike prior digital cameras, recent
cameras have resolution superior to video and the card media can store close
to 1,000 high quality images. The initial costs of the equipment are lower
and the images can be archived. The valuable in situ time is shorter as well
as the time spent processing the images. Frame-grabbing is completely
eliminated. A monopod assures a constant distance from the substrate. This
monopod holds the camera completely vertical so there are no oblique angles.
Prior to the switch, the compatibility of the methods was assessed through
intercalibration, using both methods (video and digital still images) at a
large number of sites (30) that encompassed a wide range of coral cover.
Once the methods proved
compatible, all subsequent surveys were conducted
with digital cameras.
Since 2004 high resolution digital images are taken along a 10 m transect
using an Olympus 5050 zoom digital camera with an Olympus PT050 underwater
housing. NiteRider
underwater dive lights are mounted to a cross piece to
provide consistent lighting at depths deeper than 5 m or in conditions with
poor visibility. The camera is mounted to an aluminum monopod frame, 1.7 m
from the substrate to provide a 50x69 cm image. A 6 cm bar provides a
measurement scale.
Kuulei Rodgers running a digital monopod survey. Photo
by Paul Jokiel.
The
photographer follows the following procedure:
Both
landmarks and GPS are used to relocate a site. In many cases the use of
landmarks is faster and more convenient than using the GPS position to
relocate the transect site. The diver proceeds to the start of the first 10
m transect and photographs the transect number on the clip used to mark the
site. These clips have been placed by another diver laying out the
transects. Hand signals in front of the camera (number of fingers
representing transect number as used in American sign language) are also
used to avoid any confusion during analysis. The photographer then takes 20
non-overlapping images along each 10 m transect. Each of the 10 transects
along the 100 m spine line is recorded in this manner.
Laboratory
Data Analysis
Overview
The
software program PhotoGrid is used to quantify percent cover, richness
and diversity of corals, algal functional groups and substrate cover. Images
are downloaded and the 20 non-overlapping images from each 10 m transect are
imported into PhotoGrid where 50 randomly selected points are projected onto
each image for a total of 1,000 points per transect. These data are saved in
a comma separated values (CSV) file, proofread in Excel and imported into
Microsoft Access XP, a relational database. Access data can then be queried
and exported to statistical programs for analyses. The statistical data
analysis includes a repeated measures ANOVA design with nesting of transects
in depth where frames per transect are treated as sub-samples along a
transect.

This example of the PhotoGrid screen shows the 2 components
for the data analysis. On the bottom are the identifying codes for the
Hawai‘i version of this software. It is divided up into coral species and
non-coral substrate types. The individual doing the analysis goes through
the 50 randomly generated points on the image to the right and identifies
the coral or substrate type under each intersection. These values are then
recorded on the list in the left portion of the screen and later written to
a file. (Click for a larger view)
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